What are your main challenges in small RNA analysis?
Would you prefer to have more time to work on all your scientific ideas?
Are you tired of tedious gel extraction and wasting your sequencing reads on non-functional RNAs? Does cost matter to you?
NEW TraPR kit for the isolation of functional small RNAs
TraPR (Trans-kingdom, rapid, affordable Purification of RISCs) is an easy and fast (just 15 min) single column purification method for elution of RNA-induced silencing complexes (RISCs), eliminating non-functional RNAs from the sample. The functional sRNAs can be further isolated by phenol / chloroform extraction.
- Isolate only fully functional, physiologically relevant silencing sRNAs
- Save time by using an easy single-column 15-minute workflow
- Extract high-quality sRNA even from challenging samples
- Screen for novel sRNAs in uncharacterized samples
- Simplify downstream analysis and save sequencing cost
- Targeted small-RNA analyses (qRT-PCR, RNA Blotting)
- sRNA microarrays
- Global analysis by NGS (e.g. Lexogen’s small-RNA-Seq Library Prep Kit)
- Detection and quantification of functional sRNAs, including isoforms
- Differential sRNA expression analysis (e.g. alternative to sRNA microarrays)
- Screening for novel regulatory sRNAs
- Biomarker research, discovery, and disease monitoring
TraPR delivers high sRNA specificity while saving up to 2 working days
Compared to AGO Co-Immuno-Precipitation (Co-IP) which delivers similar specificity for functional sRNAs but takes 1-2 days, TraPR saves at least 8 hours of working time. While commercially available sRNA extraction kits (using spin columns) are fast and easy, they do not confer specificity for functional sRNAs. Subsequent size-dependent methods as gel extraction to increase sensitivity are tedious and add 2 working days.
Do not waste your sequencing reads anymore and omit tedious gel extraction!
The TraPR Small RNA Isolation Kit is perfectly suited for Next Generation Sequencing (NGS) applications.
The TraPR method has been developed by Olivier Voinnet‘s group at the Department of Biology at ETH Zurich, Switzerland and was published last week in the journal Nucleic Acid Research.
Original publication: Grentzinger, T., Oberlin, S., Schott, G., et al. (2020) A universal method for the rapid isola¬tion of all known classes of functional small RNAs. Nucleic Acids Res. doi: 10.1093/nar/gkaa472