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Transfection Reagents

Transfection Reagents

Efficient transfections with low-toxicity TransIT® reagents

Use Reagent Agent® to search for the best Mirus Bio product for your transfections or to find citations for various experimental set-ups.

Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza Authors: Timothy R. Abbott, Girija Dhamdhere, Yanxia Liu, et al., Journal: Cell, Volume 181, Issue 4, 14 May 2020, Pages 865-876.e12

 

Stem Cell Transfection

Mirus Bio has several solutions for successful transfection of stem cells and other hard-to-transfect cell types.

Human adipose-derived adult stem cells upregulate palladin during osteogenesis and in response to cyclic tensile strain, Authors: Michelle E Wall, Andrew Rachlin, Carol A Otey, Elizabeth G Loboa, Journal: Am J Physiol Cell Physiol

 

Gene Editing

Mirus Bio’s TransIT-X2® is a dynamic transfection system for the delivery of CRISPR/Cas9 components (DNA, guideRNA or RNP) to mammalian cells.

Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer, Authors: Caroline Mahendra, Kathleen A Christie, Beatriz A Osuna, et al., Journal: Nature Microbiology (2020) 5:620

Horizon Discovery’s DharmaFECT Duo provides highly effective delivery of siRNA, microRNA or crRNA reagents with plasmid. Useful for experiments utilizing reporter genes, RNAi rescue, or Edit-R Cas9 plasmid.

Virus Production

Mirus Bio’s virus production reagents ensure high virus titers and easy production scale-up. TransIT-Lenti® is optimal for Lentivirus production in HEK 293T and HEK-293F cells. Choose TransIT-VirusGEN®, if your aim is to produce AAV or Lentiviruses in adherent (HEK-293T) or suspension HEK-293F cells.

Distinct Notch1 and BCL11B requirements mediate human γδ/αβ T cell development, Authors: Anne‐Catherine Dolens, Kaat Durinck, Marieke Lavaert, et al., Journal: EMBO Reports (2020) 21:e49006

 

Electroporation

Mirus Bio’s Ingenio® electroporation device, solution and cuvettes are ideal for efficient delivery of nucleic acids to difficult to transfect cell types such as primary cells and stem cells. Ingenio® solution is compatible with most electroporation devices like: Ingenio® EZporator®, Lonza-Amaxa®, Bio-Rad® and Harvard BTX®.

Kinetics of receptor tyrosine kinase activation define ERK signaling dynamics, Authors: Anatoly Kiyatkin, Iris K van Alderwerelt van Rosenburgh, Daryl E Klein, et al., Journal: Science Signaling (2020) 13:n/a