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Looking for ribosomal RNA (rRNA) depletion solution?

After extraction total RNA comprises large amounts of ribosomal RNA (rRNA) which frequently is of little interest. Ribosomal RNA (rRNA) can represent up to 90% of sequencing data, which reduces informative reads and increases sequencing costs. When depleting the rRNA you can increase dynamic range, reduce sequencing costs and simplify data analysis
– More data with less sequencing!

We offer you 2 complete rRNA depletion solutions, suited for Next Generation Sequencing NGS as well as other demanding RNA analysis applications:

1. Lexogen RiboCop


RiboCop rRNA Depletion Kit V1.2 (Human/Mouse/Rat)

  • Efficient elimination of cytoplasmic and mitochondrial rRNA – sequencing depth is preserved for relevant RNA transcripts
  • Simple 2h workflow – no enzymatic reactions or mechanical shearing
  • Output ready for NGS library prep
  • Down to 1 ng of total RNA input, up to 1ug
  • Cost-efficient solution – do not waste any extra money for this routine RNAseq step
  • Available as 24rxn and 96rxn stand-alone kit kits, as well as in combination with SENSE Total RNA-Seq Library Prep Kit 
  • Easy for you; all needed reagents included
  • Ask for 4 prep trial!

 

2. NuGEN AnyDeplete – A unique method for eliminating unwanted reads in RNA-Seq libraries

  • AnyDeplete provides consistent depletion of unwanted reads after library preparation, eliminating bias and the need for higher sample input
  • When depletion is done on the library level, it is saving your sample and the RNA integrity – lower bias
  • AnyDeplete is integrated with NuGEN RNA-Seq library preparation kits which are available for input levels from a single cell (10 pg) to 1 μg to provide a streamlined solution
  • With AnyDeplete you can choose standard rRNA depletion from human, mouse, bacteria, Drosophila, and other model organisms
  • You can also customize AnyDeplete to remove unwanted rRNA from your organism of interest and any unwanted transcript -Thus applicable to any species!