Panexin Seeruminkorvike PAN-Biotechilta
Ei enää seerumin testausta! Panexin basic -seerumikorviketta voidaan käyttää adherentti- ja suspensiosolujen kasvatukseen seerumivapaissa olosuhteissa, tai vähentää huomattavasti tarvittavaa seerumimäärää kasvatuksessa.
Vaativimmille solulinjoille on varta vasten suunniteltu Panexin NTA (adherentit solut), NTS (suspensiosolut) ja Panexin Pharma Grade (vapaa eläinperäisistä komponenteista).
Panexin- Chemically defined serum replacement
Easy to use:
Panexin basic can be stored and used in the same manner as serum. The performance can be further improved by optimizing the concentration of Panexin or modifying/changing the basal medium. IMPORTANT: If Trypsin is used to detach adherent cells it needs to be deactivated with Trypsin inhibitor (1 ml inhibitor per 1 ml Trypsin). Accutase does not need to be inhibited.
to reduce serum
Media with 10% FBS:
FBS contains hundreds of distinct proteins and thousands of metabolites in undefined, varying concentrations. Resulting in inconsistent results and unreproducible data. Data in figure from M. Baker, Nature 537 2016.
Media with 1% FBS and 9% Panexin:
The variation of bioactive components in FBS from lot to lot can be reduced tremendously. Can be easily adaped to a wide range of cell types. With significantly improved reproducibility. More independent from the lot, the origin and the supplier of FBS.
Serum-free media with Panexin:
Constant quality
Highest reproducibility
No more serum testing!
Applications
As serum replacement or medium supplement to increase the productivity in industrial cell cultures (CHO, MDCK, Vero, Hybridoma etc.).
To avoid the exosoms or stimulatory effects of growth factors in serum.
To prevent the overgrowth of the culture by fibroblasts in coculture or in highly differentiated epithelial primary cultures.
To guarantee the reproducibility and sensitivity in cell-based in vitro assays.
To generally reduce the amount of serum due to ethical concerns, lot-to-lot variability or high costs.
Advantages
- High reproducibility
- No extensive batch testing necessary
- Simplified downstream process
- Low risk of contamination
- Design your own defined serum-free or serum-reduced medium!
Lue lisää PAN-Biotechin esitteestä
Seerumikorvikkeet ovat tasalaatuisia, eräkohtaisia eroja ei ole – näin vältät seerumien erätestailut. Kysy ilmainen näyte kokeiluusi!