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DNAseq solutions

For your need of whole genome sequencing (WGS), exome sequencing (WES), metagenomics, hybridization capture, ChIP-seq or any other DNA sequencing applications, we offer several solutions:

For low input < 1ng, picogram levels of DNA:

ACCEL-NGS® 2S PLUS DNA LIBRARY KIT (Swift Biosciences). Versatile DNA Library Preparation from limited samples, ChIP-Seq. Utilizes a proprietary adapter attachment chemistry which minimizes bias and supports inputs as low as 10 pg.

Ovation® Ultralow V2 DNA-Seq Lib Prep Kit (NuGEN/Tecan Genomics). Simple DNA-Seq library preparation workflow for low input and degraded sample types, input 10 pg – 100 ng. Including enzymatic fragmentation, UDIs and UMIs – Superior lib prep solution for e.g. liquid biopsy/cfDNA samples.

NxSeq UltraLow DNA Library Kit v2 (Lucigen/LGC Biosearch). Cost-efficient choice for inputs starting 50pg. High efficiency adaptor ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer missed regions.

For highly degraded, ancient, single-strand DNA samples:

Accel-NGS® 1S DNA Library Kits: Will yield libraries in 2 hours from precious, damaged or degraded samples (ancient, FFPE), using as little as 10 pg of input, or fragments as short as 40 bp. Leveraging the kit’s unique, patented Adaptase® chemistry, a highly efficient template-independent adapter ligation strategy, you are sure to gain insights from samples that are unusable by other existing methods. Produces High Quality Cut&Run Data.

For regular DNA sequencing applications, the best choice:

SWIFT 2S® TURBO DNA LIBRARY KITS

The Easiest NGS Workflow for Routine Sequencing, with enzymatic fragmentation. Simple, fast (2h) and reliable. Compatible with diverse genome types of low or high complexity. One universal approach for whole genome, exome, and large gene studies. Compatible with several indexing options such a Single, Combinatorial Dual (up to 768-plex) or Unique Dual Indexing UDIs. Read more

Easily automatable – Several scripts already available!

Accel-NGS 2S PCR-Free DNA Library Kit: Eliminate Sequencing Bias from Polymerase with PCR-free NGS Prep. With unbiased coverage from cfDNA inputs as low as 10 ng and sheared DNA as low as 100 ng.

Accel-NGS 2S Hyb DNA Library Kit: NGS Prep for Hybridization Capture workflows. Produces fewer duplicates at 1 ng to save you money on sequencing costs. A variety of indexing kits allow for compatibility with multiple hybridization capture technologies: Swift Hybridization Capture Kits, IDT® xGen®Lockdown® Probes, Twist Human Core Exome Kit and Agilent SureSelect. This kit is the right choice for researchers working with applications pertaining to low input FFPE DNA or whole genome sequencing of liquid biopsy and cfDNA samples.

 

SWIFT BIOSCIENCES NORMALASE

Revolutionary enzymatic NGS library normalization technology that streamlines library balancing and pooling for ease of loading on Illumina® sequencing platforms. Compatible with indexing by PCR and indexing by ligation for both DNA and RNA library prep workflows.

Read more

 

TECAN GENOMICS – NuGEN

Tecan offers simple and streamlined workflows for DNA sequencing library preparation on Illumina platforms. Includes proprietary DimerFree® technology – that simplifies the
workflow by eliminating adaptor dimers and adaptor dilution steps – and NuQuant® for rapid, integrated library QC in less than six minutes for research purposes.

Celero™ DNA-Seq: short, three-step, addition-only, single-tube library preparation. Only one bead-purification. Available also with enzymatic fragmentation & UDIs

Celero EZ DNA-Seq Lib Prep Kit: A simple 3-step workflow integrated with NuQuant library quantification that provides a flexible solution for high-throughput DNA-Seq. With enzymatic fragmentation technology that is simple, robust and consistent for fragmentation of genomic DNA into homologous sized fragments, without optimization. Eliminates post-ligation bead purification, resulting in faster library preparation and reduced hands-on time.

Rapid EZ DNA-Seq: fast, PCR-free DNA-Seq lib prep workflow with enzymatic fragmentation that can be easily completed in under two hours. Easily automatable.

To help you choose the right option for your experiment, check the DNAseq Selection Guide!

 

Check all our NGS solutions from here!