RNA sequencing (RNA-Seq) is a powerful NGS tool with a variety of applications, including gene expression analysis, whole-transcriptome profiling, the identification of novel transcripts, the detection of coding variants, the detection of fusion genes, and the identification of alternative splicing, among others.
The benefits of the Immuno Diagnostic RNAseq solution portfolio includes:
- Easier & faster protocols (most doable within couple of hours, one lab day!)
- Lower RNA input options
- More cost-effective sequencing, which is obtained with of course better lib prep pricing and more specific & effective lib prep performance
All-inclusive fast and cost-efficient technologies for transcriptome analysis.
Whole Transcriptome Library Prep CORALL – Complete coverage of transcripts from start to end (incl. UMIs & UDIs). Combine with rRNA depletion to obtain data from total-RNA status, including non-coding RNAs. 1 ng to 1 µg of total RNA input.
mRNAseq analysis: CORALL + poly-A-selection bundle. Fast and cost-efficient generation of stranded, UMI labelled, and unique dual indexed libraries for whole transcriptome poly(A) RNA analyses.
QuantSeq 3´ mRNA-Seq: THE GOLD STANDARD for genome-wide analysis of gene expression. Cost-efficient alternative to microarrays and standard RNA-Seq. Together with UMIs & UDIs. Free data analysis pipeline on the Bluebee® platform included.
QuantSeq-POOL 3’ mRNA-Seq Lib Prep Kit: Optimal solution for gene expression profiling for large screening projects using sample barcoding, early pooling, and batch processing of up to 96 samples in one reaction providing a workflow that is easily scalable for multiplexing up to 36,864 samples.
QuantSeq-FLEX Targeted RNA-Seq – Targeted sequencing with custom-designed gene-specific primers
LUTHOR 3’ mRNA-Seq Lib Prep Kit: For single-cell analysis. Combines a novel direct RNA amplification technology with an efficient one-step 3’ RNA-Seq library preparation method yielding unprecedented sensitivity and reproducibility for individual cells and purified RNA in the ultra-low pg range.
SLAMseq Metabolic RNA Labeling: Option for e.g. GRO-seq – Analyze transcriptome-wide kinetics of RNA synthesis and turnover and measure nascent RNA expression and transcript stability
Small RNA-Seq Library Prep Kit for miRNA analysis. Gel-free protocol. Optimized for miRNA studies & low RNA content samples such as plasma, serum, and urine, wide input range starting from 50 pg
RiboCop rRNA Depletion Kit (Human/Mouse/Rat V2): Removes cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences. The most efficient method in the market to get rid of ribosomal RNA -> Saves your sequencing costs!
RiboCop HMR + Globin: The best solution for whole blood sample RNAseq; you can deplete both rRNA and globin mRNA in a single step -> providing a highly convenient workflow and freeing up sequencing space for RNAs of interest.
RiboCop for Bacteria META: The optimized solution to deplete bacterial rRNA from e.g. stool and environmental samples. Combine with Corall to create the comprehensive solution for metatranscriptomics study!
Poly(A) RNA Selection Kit V1.5: Enables rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.
Swift RNA Library Kit & Swift Rapid RNA Library Kit offer a fastest, lowest cost robust NGS transcriptomics workflow with optimal transcript coverage and NGS data quality from a broad range of input quantities for Illumina® sequencing platforms.
Leveraging patented Adaptase® technology, this kit enables stranded RNA library
construction directly from 1st strand cDNA without the requirement for 2nd strand cDNA synthesis and degradation, or template-switching methods. The kit is compatible with manual and automated workflows as well as upstream and downstream enrichment and depletion methods and supports a variety of indexing options.
TECAN GENOMICS – NuGEN
These solutions feature novel, proprietary technologies, including:
- AnyDeplete® targeted, customizable transcript depletion – to efficiently remove unwanted transcripts
- Single Primer Isothermal Amplification (SPIA®) – for detection of rare transcripts
- DimerFree adaptor ligation
- NuQuant for rapid, integrated library quantification
Use the RNA-Seq selection guide to find the right kit for your application.
NEW: Revelo™ RNA-Seq library preparation kit – High Sensitivity RNA-Seq library preparation kit. One-day sample to sequencer solution for pathogen detection. Most commercially available RNA-Seq kits are inadequate to sensitively detect and characterize low-titer viral or other pathogenic agents from samples in high human background, such as nasal swab samples SARS-CoV-2 infected patients. Revelo RNA-Seq is a whole transcriptome solution optimized for the detection and characterization of rare and low abundance transcripts from degraded samples, with inputs as low as 250 pg.
Universal Plus mRNA-Seq with NuQuant – streamlined library preparation solution for mRNA sequencing. Integrated globin depletion for blood sample RNAseq.
Universal RNA-Seq with NuQuant: First end-to-end solution for whole transcriptome RNA-Seq libraries combining library preparation, targeted depletion and library quantification. Includes species-specific rRNA depletion for Human, Mouse, Drosophila, Arabidopsis, CUSTOM – For any species of interest!
Universal Prokaryotic RNA-Seq: Simple solution for metatranscriptomic studies. An easy-to-use add and incubate system ideal for microbiological RNA sequencing studies, complete solution for strand-specific RNA-Seq library construction using total RNA obtained from pure cultures or mixed populations of bacteria. The integrated AnyDeplete technology enables rRNA depletion from a broad range of prokaryotes optimizing informative reads and reducing sequencing costs.
Ovation SoLo RNA-Seq: The ONLY single cell whole transcriptome solution in the market, with integrated rRNA depletion for Human, Mouse, Drosophila. Robust library preparation RNAseq workflow from single cells (1-500) or ultralow, degraded inputs starting from 10 pg.
Trio RNA-Seq: complete solution for library preparation, incorporating SPIA amplification method for sensitive detection of rare, low expression transcripts e.g. virus studies
Ovation RNA-Seq System V2: rapid and sensitive workflow for generating micrograms of cDNA for multiple downstream applications