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Reverse transcription and cDNA synthesis

High quality solutions for cDNA synthesis

No matter what your sample type or species is, or if you are performing qPCR or NGS, check these  interesting options:

1. cDNA synthesis from single cells or low input samples: CelluLyser lysis and cDNA Synthesis kit from TATAA

  • From cells to cDNA in one tube: CelluLyser lysis buffer combined with highly efficient GrandScript cDNA Synthesis Kit
  • Sensitive and easy to use
  • By using CelluLyser™,  the entire workflow from rapid and sensitive cell lysis to cDNA synthesis and qPCR can be performed without washing steps, thus eliminating material loss
  • Suitable for samples ranging from 10 000 cells down to as little as one single cell.

 

2. Whole transcriptome RNA amplification process for low input & degraded samples: NuGEN/Tecan Genomics Ovation® RNA-Seq System V2

NuGEN/Tecan Genomics Ovation® RNA-Seq System V2 provides a fast and simple method for preparing amplified cDNA from total RNA for multiple downstream applications including RNA-Seq, qPCR and archival storage for future analyses.

  • Powered by Ribo-SPIA® technology, a rapid, simple and sensitive RNA amplification process developed by NuGEN
  • Using Ribo-SPIA technology and starting with as little as 500 pg total RNA, microgram quantities of cDNA can be prepared
  • Ribo-SPIA contributes minimal coverage bias which has been shown to be highly reproducible
  • After purification, the cDNA can be fragmented to the appropriate size and ligated into NuGEN or other suitable NGS library construction methods

 

3. For generating long cDNA transcripts: Lexogen TeloPrime

  • The TeloPrime Full-Length cDNA Amplification Kit V2 is an all-in-one protocol for generating full-length cDNA from total RNA.
  • Full-length cDNA generation with high yield
  • Exceptional 5‘ cap specificity
  • Ideal for long-read NGS library generation (e.g. PacBio™ and Oxford Nanopore™)
  • 1 ng – 2 μg total RNA input
  • Flexible protocol enables use of custom primers for reverse transcription

 

4. For production of full-length cDNA from low amounts of total RNA: MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit

  • Optimized, complete system for production of full-length cDNA from low amounts of total RNA.
  • Synthesize full-length cDNA (>15 kb)
  • Includes all components necessary to generate first-strand cDNA from picogram amounts of total RNA
  • MMLV HP RT demonstrates significantly higher activity than competitive reverse transcriptase enzymes

 

5. MessageBOOSTER™ cDNA Synthesis Kit for qPCR

  • Produce amplified amounts of cDNA from precious, limiting total RNA samples without introducing bias.
  • Perform RNA amplification on purified total RNA followed by cDNA synthesis and detection
  • Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample.
  • Get more data out of precious samples – use less RNA for more RT-qPCR reactions
  • Readily and reproducibly detect even low-abundance transcripts in RNA from a single cell (CT values <35 cycles)
  • Collect small RNA samples less often.
  • Fast protocol amplifies and synthesizes cDNA in only 1 day.

 

6. Crescendo cDNA™ Synthesis for qPCR

  • Whole transcriptome approach allows qPCR for any target
  • Increased sensitivity of detection of target genes
  • Generate large amount of cDNA for multiple applications
  • Compatible with limited RNA quantity and quality -input range 500 pg – 50 ng total RNA
  • Kit sizes: 16 & 64 rxn

 

Reverse transcriptase Enzymes

1. EpiScript™ RNase H- Reverse Transcriptase

  • Cost-efficient replacement for SuperScript reverse transcriptase. The working conditions are similar, performance better!
  • Produce full-length cDNA with RNAse H- mutant
  • Achieve higher specificity at elevated temperatures, up to 55°C
  • Part of the RapiDxFire 1-step RT-qPCR system for effective detection of viral RNA pathogens.

 

2. MMLV High Performance Reverse Transcriptase

  • Optimized reverse transcriptase and buffer system for the production of full-length cDNA (>15 kb)
  • Amplify first-strand cDNA from picogram amounts of total RNA
  • Optimize the RT reaction to your specific needs by using the first strand cDNA synthesis primers, dNTPs and RNase inhibitor of your choice (not included).

 

3. NxGen® M-MuLV Reverse Transcriptase

  • Outstanding cDNA synthesis
  • RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

 

4. RapiDxFire™ Thermostable Reverse Transcriptase

  • A truly thermostable reverse transcriptase for fast synthesis of short cDNA (< 1 Kb)
  • A significant advancement over popular MMLV- and AMV reverse transcriptases
  • Extremely active at high temperatures (55 to 80°C): Improves specificity of cDNA Synthesis from diverse RNA templates
  • Sensitive: Detects ≤100 copies of RNA in two-step RT-qPCR assays
  • Short reaction time (5 minutes or less): Streamlined RT-qPCR workflow and faster time to results
  • Stable at room temperature (> 3 months): Simplifies setup on automation decks (no cold storage required) and enables use in environments with limited cold-storage
  • Lyo-compatible: Enzyme formulation is free of glycerol -and other components- that are known to interfere with downstream lyophilization
  • Batch to batch reproducibility: Manufactured in an ISO 13485-certified facility
  • Bulk/OEM available: Contact us about bulk orders, custom formulations, and dispensing options.