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Methyl-Seq Epigenetics

Epigenetics is the study of functional modifications of the genome that do not change the DNA sequence. These modifications determine cellular differentiation, growth, disease progression and therapeutic response. E.g. environmental factors changes the epigenome, which genes are turned on/off and thus regulate gene expression and genome organization.

To investigate this phenomenon further, we offer several tools for your research:

  • DNA methylation sequencing methods
  • Whole genome bisulfite sequencing (WGBS)
  • Reduced representation bisulfite sequencing (RRBS)
  • Targeted bisulfite sequencing through hybridization capture methods (also as a service!)
  • Oxidative bisulfite sequencing (oxBS-Seq, to distinguish between 5mC and 5hmC)
  • Single-cell methylation solution

Swift Biosciences’ Methyl-Seq Products

Swift Biosciences offers solutions for whole genome bisulfite sequencing as well as targeted bisulfite sequencing applications, such as RRBS and hybridization capture.

The GOLD STANDARD: Accel-NGS Methyl-Seq DNA Library Kit

For single-base resolution of methylomes. Provides the most comprehensive coverage of the methylome (presented at Nature Reports 2019). The Adaptase® chemistry enables the construction of high complexity libraries and supports very low DNA input amounts due to high recovery of bisulfite-converted DNA.

For SINGLE CELL METHYLATION SEQUENCING: Accel-NGS Adaptase Module

  • Constructs NGS libraries from bisulfite-converted, single-stranded DNA from single cells
  • Optimized to maximize the recovery of DNA
  • Three-dimensional indexing for high-throughput sequencing 384-plex
  • Described in Science paper

 

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NEW! Target Your regions of interest:

CUSTOM Methylation Sequencing with Daicel Arbor Biosciences myBaits Hybridization Capture panels

Enables you to efficiently enrich target genes and markers of interest from converted methyl-seq NGS libraries. The hybridization capture technique significantly reduces per-sample sequencing costs compared to whole-genome bisulfite sequencing (WGBS) while preserving the essential strand-specific methylation signals.

myBaits Custom Methyl-Seq kits can be paired with either bisulfite or enzymatic-conversion workflows upstream of NGS library preparation, for seamless integration into your existing methyl-seq pipeline. All kits include the latest “v5” generation of myBaits hybridization and wash reagents, pairing best-ever performance with an easy-to-use protocol.

NuGEN / Tecan Genomics Methyl-Seq solutions

Comprised of library preparation and bisulfite conversion tools for a broad range of applications for NGS, methylation arrays, and more. Now, with the integration of TrueMethyl® oxBS chemistry, gain new insight into epigenetic modifications with the ability to interrogate both 5mC and 5hmC.
The oxBS technology enables the most accurate 5-methylcytosine (5mC) identification for NGS, and the interrogation of 5-hydroxymethylcytosine (5hmC), a modified base that is not assayed by traditional bisulfite conversion approaches. Download the Selection Guide! 

Products:

  • Ultralow Methyl-Seq with TrueMethyl oxBS. Methylation detection from as little as 10 ng input enables studies of samples such as cfDNA. All-in one DNAseq lib prep solution, to distinguish the two types of methylation.
  • Ovation® RRBS Methyl-Seq with TrueMethyl® oxBS. Inclusion of diversity adaptors eliminates the need for PhiX spike-in, reducing sequencing costs without sacrificing data. Includes enzymatic fragmentation and molecular tags.
  • TrueMethyl oxBS2 module also available separately

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Check all our NGS solutions from here!