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Competent cells, Cloning and Enzymes

Competent cells

GENERAL CLONING: High efficiency cells results in high colony counts.
Use LGC´s high efficiency E. cloni ® 10 G and 10 GF´ chemically competent and electrocompetent cells to replace standard cloning strains such as DH5alpha™, DH10B™, JM109, TOP10, XL1-Blue etc.

CRISPR LENTIVIRAL GUIDE LIBRARIES:
Endura™ competent cells
for repetitive sequences are recommended in CRISPR GeCKO library protocols.

COMPETENT CELLS SELECTION GUIDE:

Download the Competent Cell Selection Guide to make it easier to choose the right cell line for your needs.

Cloning

GENERAL CLONING: CloneSmart® Cloning Kits

  • Pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation
  • Transcription terminators flanking cloning site stabilize otherwise toxic inserts
  • Choice of copy number and antibiotic resistance
  • Use with E. cloni® 10G electrocompetent or chemically competent cells

 

DIFFICULT CLONING OR BAC/FOSMID CLONING: BigEasy® v2.0 Linear Cloning System

  • Maximum insert stability
  • Efficiently clone any insert up to 30 kb
  • Create libraries from A/T-rich or G/C-rich genomes
  • Clone gene clusters or operons
  • Inducible copy number

 

Recombineering with Red/ET

Recombineering with Red/ET allows cloning, subcloning and modification of DNA at any chosen position. It permits engineering of DNA molecules of any size, including BACs and the E. coli chromosome.

Red/ET Recombination increases the power of CRISPR/Cas9 to extend genome studies. By combining CRISPR/Cas9 with Red/ET Recombineering Gene Bridges successfully knocked in a 33 kb human fragment into the mouse ApoE locus. Read published work for more details.

PCR cleanup & plasmid DNA purification

We offer PCR cleanup kits and plasmid DNA purification kits e.g. from Geneaid. Check Geneaid’s product list here.

Enzymes

LGS´s Enzymes are selected to meet your molecular biology and NGS needs:

DNA polymerases e.g. NxGen® phi29 DNA polymerase, Exo-Minus Klenow DNA polymerase, FailSafe™ enzyme, EconoTaq® DNA polymerase.
RNA polymerases e.g.  NxGen® T7 RNA polymerase, T7 R&DNA™ polymerase
Reverse transcriptases e.g. MMLV High Performance reverse transcriptase, NxGen® M-MuLV reverse transcriptase, EpiScript™ RNase H- reverse transcriptase.
DNA endonuclease: Baseline-ZERO™ DNase & RNase-free DNase
DNA exonucleases: exonuclease I, III ja VII ja Plasmid-Safe™ ATP-Dependent DNase.
RNA nucleases: e.g. RNase A, I & R
DNA/RNA nucleases: e.g. Terminator™ 5′-Phosphate-Dependent exonuclease
Lysozyme: Ready-Lyse™ lysozyme
Ligases: e.g.  NxGen® T4 DNA Ligase ja CircLigase® ssDNA Ligase
Fosfatases & kinases: e.g. RNA 5′ polyfosfatase, T4 polynucleotide kinase
Endonucleases (RNA-guided): e.g. CRISPRcraft™ S.p. Cas9 nuclease
RNase-inhibitors: e.g. NxGen® RNase inhibitors

Available also in bulk volumes for heavy users. Read more from the Enzymes for molecular biology catalog.

Enzyme catalog