GENERAL CLONING: High efficiency cells results in high colony counts.
Use LGC´s high efficiency E. cloni ® 10 G and 10 GF´ chemically competent and electrocompetent cells to replace standard cloning strains such as DH5alpha™, DH10B™, JM109, TOP10, XL1-Blue etc.
CRISPR LENTIVIRAL GUIDE LIBRARIES:
Endura™ competent cells
for repetitive sequences are recommended in CRISPR GeCKO library protocols.
COMPETENT CELLS SELECTION GUIDE:
Download the Competent Cell Selection Guide to make it easier to choose the right cell line for your needs.
GENERAL CLONING: CloneSmart® Cloning Kits
- Pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation
- Transcription terminators flanking cloning site stabilize otherwise toxic inserts
- Choice of copy number and antibiotic resistance
- Use with E. cloni® 10G electrocompetent or chemically competent cells
DIFFICULT CLONING OR BAC/FOSMID CLONING: BigEasy® v2.0 Linear Cloning System
- Maximum insert stability
- Efficiently clone any insert up to 30 kb
- Create libraries from A/T-rich or G/C-rich genomes
- Clone gene clusters or operons
- Inducible copy number
Recombineering with Red/ET
Recombineering with Red/ET allows cloning, subcloning and modification of DNA at any chosen position. It permits engineering of DNA molecules of any size, including BACs and the E. coli chromosome.
Red/ET Recombination increases the power of CRISPR/Cas9 to extend genome studies. By combining CRISPR/Cas9 with Red/ET Recombineering Gene Bridges successfully knocked in a 33 kb human fragment into the mouse ApoE locus. Read published work for more details.
PCR cleanup & plasmid DNA purification
We offer PCR cleanup kits and plasmid DNA purification kits e.g. from Geneaid. Check Geneaid’s product list here.
LGS´s Enzymes are selected to meet your molecular biology and NGS needs:
DNA polymerases e.g. NxGen® phi29 DNA polymerase, Exo-Minus Klenow DNA polymerase, FailSafe™ enzyme, EconoTaq® DNA polymerase.
RNA polymerases e.g. NxGen® T7 RNA polymerase, T7 R&DNA™ polymerase
Reverse transcriptases e.g. MMLV High Performance reverse transcriptase, NxGen® M-MuLV reverse transcriptase, EpiScript™ RNase H- reverse transcriptase.
DNA endonuclease: Baseline-ZERO™ DNase & RNase-free DNase
DNA exonucleases: exonuclease I, III ja VII ja Plasmid-Safe™ ATP-Dependent DNase.
RNA nucleases: e.g. RNase A, I & R
DNA/RNA nucleases: e.g. Terminator™ 5′-Phosphate-Dependent exonuclease
Lysozyme: Ready-Lyse™ lysozyme
Ligases: e.g. NxGen® T4 DNA Ligase ja CircLigase® ssDNA Ligase
Fosfatases & kinases: e.g. RNA 5′ polyfosfatase, T4 polynucleotide kinase
Endonucleases (RNA-guided): e.g. CRISPRcraft™ S.p. Cas9 nuclease
RNase-inhibitors: e.g. NxGen® RNase inhibitors
Available also in bulk volumes for heavy users. Read more from the Enzymes for molecular biology catalog.