Competent cells, Cloning and Enzymes

Competent cells

GENERAL CLONING: High efficiency cells results in high colony counts.
Use LGC´s high efficiency E. cloni ® 10 G and 10 GF´ chemically competent and electrocompetent cells to replace standard cloning strains such as DH5alpha™, DH10B™, JM109, TOP10, XL1-Blue etc.

CRISPR LENTIVIRAL GUIDE LIBRARIES:
use cells that are recommended in CRISPR GeCKO library protocols and choose LGC´s  Endura™ competent cells for repetitive sequences.

 

Cloning

GENERAL CLONING: CloneSmart® Cloning Kits

  • Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation
  • Transcription terminators flanking cloning site stabilize otherwise toxic inserts
  • Choice of copy number and antibiotic resistance
  • Optimized for use with E. cloni® 10G electrocompetent and chemically competent cells

 

DIFFICULT CLONING OR BAC/FOSMID CLONING: BigEasy® v2.0 Linear Cloning System

  • Maximum insert stability
  • Efficiently clone any insert up to 30 kb
  • Create libraries from A/T-rich or G/C-rich genomes
  • Clone gene clusters or operons
  • Inducible copy number

 

Recombineering with Red/ET

Recombineering with Red/ET allows cloning, subcloning and modification of DNA at any chosen position. It permits precise engineering of DNA molecules of any size, including very large ones such as BACs or the E. coli chromosome.

Red/ET Recombination increases the power of CRISPR/Cas9 to extend genome studies. By combining CRISPR/Cas9 with Red/ET Recombineering Gene Bridges successfully knocked in a 33 kb human fragment into the mouse ApoE locus. Read published work for more details.

PCR cleanup & plasmid DNA purification

We offer PCR cleanup kits and plasmid DNA purification kits e.g. from Geneaid. Check Geneaid’s product list here.

Enzymes

LGS´s Enzymes are selected to meet your molecular biology and NGS needs:
ampligases, circligases, nucleases, transposases, polymerases, reverse transcriptases,
RNase inhibitors etc. Available also in bulk volumes for heavy users. Enzymes for molecular biology catalog

DNA polymerases e.g. NxGen® phi29 DNA polymerase, Exo-Minus Klenow DNA polymerase, FailSafe™ enzyme, EconoTaq® DNA polymerase.
RNA polymerases e.g.  NxGen® T7 RNA polymerase, T7 R&DNA™ polymerase
Reverse transcriptases e.g. MMLV High Performance reverse transcriptase, NxGen® M-MuLV reverse transcriptase, EpiScript™ RNase H- reverse transcriptase.
DNA endonuclease: Baseline-ZERO™ DNase & RNase-free DNase
DNA exonucleases: exonuclease I, III ja VII ja Plasmid-Safe™ ATP-Dependent DNase.
RNA nucleaases: e.g. RNase A, I & R
DNA/RNA nucleases: e.g. Terminator™ 5′-Phosphate-Dependent exonuclease
Lysozyme: Ready-Lyse™ lysozyme
Ligases: e.g.  NxGen® T4 DNA Ligase ja CircLigase® ssDNA Ligase
Fosfatases & kinases: e.g. RNA 5′ polyfosfatase, T4 polynucleotide kinase
Endonucleases (RNA-guided): e.g. CRISPRcraft™ S.p. Cas9 nuclease
RNase-inhibitors: e.g. NxGen® RNase inhibitors